Microscopy was done using two Molecular Devices (Sunnyvale, CA, USA) ImageXpress microscopes connected to a Thermo CRS plate loader. 9 pictures were acquired per well. Laser-based autofocus was performed at each site. Signals from two channels (Hoechst and YFP) were collected using 20x and 10x S Fluor 0.45 objectives for the primary and secondary screen, respectively. Altogether, approximately 2’045’000 images were taken for the primary screen (296 x 384-well plates, 9 images 2 channels), and 311’000 for the secondary screen (3 x 15 x 384-well plates, 9 images, 2 channels).